Deteksi dan Kuantifikasi Cemaran Babi pada Sampel Olahan Daging Menggunakan Real-time PCR

AbstrakMetode pengujian cemaran babi menjadi faktor penting dalam sertifikasi produk halal. Metode yang cepat dan robust diperlukan untuk deteksi kuantifikasi babi. Real-time PCR atau dikenal dengan istilah quantitative (qPCR) merupakan metode alternatif berdasarkan residu keberadaan DNAnya pada sampel olahan pangan. ekstraksi DNA kit amplifikasi tahan terhadap inhibitor kunci keberhasilan penggunaan qPCR pendeteksian Pendeteksian probe digunakan karena mempunyai kelebihan inhibitor, cepat, spesifik, multipel target. Penelitian ini bertujuan mendeteksi menguantifikasi menggunakan secara qPCR. Tahapan penelitian adalah DNA, amplifikasi, deteksi, Sampel berasal dari pangan, seperti bakso, sosis, daging burger, siomay, kuah daging, isi roti. Hasil menunjukkan bahwa terdapat bakso. didapatkan bakso memiliki persentase kontaminasi sejumlah 25%, sedangkan 12,5%. dapat direkomendasikan laboratorium penguji makanan sebagai pangan akurat.AbstractPork contamination testing method is an important factor in halal product certification. A fast and needed for the detection quantification of pig contamination. or commonly known as alternative pork based on pig’s residual presence processed food samples. extraction inhibitor-resistant amplification are keys successful implementation Detection with used because it has some advantages, such resistant to inhibitors, fast, specific, multiple targets. This research aimed detect quantify using rapid The stages this were extraction, amplification, detection, quantification. samples taken from products, meatballs, sausage, burgers’ meat, dumplings, meat broth, filled bread. results showed that there was broth. meatballs had a percentage while broth 12.5%. study can be recommendation laboratories detecting products quickly accurately.